Improvement of solid-state anaerobic digestion of broiler farm-derived waste via fungal pretreatment

Abstract

Fungus, Trichoderma longibrachiatum, was used for the pretreatment of broiler farm derived-lignocellulosic bedding material (rice husk) to enhance the subsequent solid-state anaerobic digestion (SS-AD). Fungal pretreatment efficacy was evaluated through a series of batch studies with respect to carbon-to-nitrogen (C/N) ratio and pretreatment time. Lignocellulosic outer layer structure disruption of the rice husk was prominent under the best fungal pretreatment condition evaluated (C/N ratio of 18.9 and pretreatment time of 7 days). Consequently, the resulting methane yield of 438.1 ± 20.0 NmL/gVSadded was obtained which was ~2.0-folds higher than that of the control (without pretreatment). Furthermore, in semi-continuous SS-AD, fungal pretreatment could significantly enhance digestibility of organic substance in high solid loading (30% total solids) AD process by 3.2-folds and improve microbial kinetic parameters with subsequent daily methane yield improvement by 2.4-folds. Thus, fungal pretreatment could be an environmentally-friendly and effective low-cost approach for broiler farm-derived waste management to enhance SS-AD efficacy.