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dc.contributor.authorUtoomporn Surayoten_US
dc.contributor.authorSutee Wangtueaien_US
dc.contributor.authorSangguan Youen_US
dc.contributor.authorSubramnaian Palanisamyen_US
dc.contributor.authorWarawut Krusongen_US
dc.contributor.authorCharles S. Brennanen_US
dc.contributor.authorFrancisco J. Barbaen_US
dc.contributor.authorYuthana Phimolsiripolen_US
dc.contributor.authorPhisit Seesuriyachanen_US
dc.description.abstractThis research aimed to extract mucilage polysaccharides (MP) from Amanita hemibapha subspecies javanica (Corner and Bas), and further fractionate them using anion-exchange chromatography, yielding two fractions (MPF1 and MPF2). The crude extract, and fractions mainly consisted of carbohydrates (83.5–93.2%) with minor amounts of proteins (5.40–7.20%), and sulphates (1.40–9.30%). Determination of the monosaccharide composition revealed that glucose was the major unit, followed by galactose, mannose, rhamnose, and arabinose. The average molecular weight (MW) of the crude extract and fractions was in the range 104.0–479.4 × 103 g/mol. Interestingly, the crude extract, and fractions did not cause any toxic effect in RAW264.7 cells. However, they stimulated the RAW264.7 cells to release nitric oxide and cytokines through the activation of nuclear factor-kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) pathways via cell surface TLR4. Structural analysis of the most immunestimulating extract fraction, MPF2, revealed that the main backbone consisted of α-D-(1→6)-glucopyranoside. These results suggest that the MPs derived from A. hemibapha subspecies javanica (Corner and Bas) are potent in enhancing immunity; hence, they can be used as a functional ingredient in food products.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleExtraction, structural characterisation, and immunomodulatory properties of edible amanita hemibapha subspecies javanica (Corner and bas) mucilage polysaccharide as a potential of functional fooden_US
article.title.sourcetitleJournal of Fungien_US
article.volume7en_US Mongkut's Institute of Technology Ladkrabangen_US National Universityen_US Universityen_US de Valènciaen_US Mai Universityen_US
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