Development and Validation of HPLC Method for Determination of Quercetin in Hydrogel Transdermal Patches Loaded with Red Onion Peel Extract

Abstract

Quercetin is the major flavonoid present in red onion (Allium cepa L.). The determination of quercetin in red onion extract has been investigated previously. However, the HPLC method for analyzing quercetin in hydrogel transdermal patches with red onion peel extract has not been reported. This study aimed to develop and validate the novel HPLC methods for the determination of quercetin in hydrogel transdermal patches loaded with red onion peel extract. The HPLC condition and system configuration were optimized to obtain acceptable validation parameters. Method validations were performed for the quantitative analyses of quercetin in hydrogel transdermal patches with red onion peel extract. The reversed phase HPLC analytical method was developed. The C18 column and 100% acetonitrile and 0.1% aqueous phosphoric acid solution as a mobile phase were applied at a flow rate of 1.0 mL/min with gradient elution. The injection volume was 10 µL. The separation was performed at ambient temperature with UV detection at 364 nm. The retention time of quercetin was 23.9 min, with a total run time of 27 min. The linearity range of calibration curves was 5-150 µg/mL (R2>0.999). The limit of detection (LOD) and limit of quantification (LOQ) were 0.18 and 0.53 µg/mL, respectively. The mean recoveries were reported as 99.35–108.29%, indicating accuracy of the method. The %RSD study was less than 2.0%, suggesting the precision of the method. This method successfully determined quercetin in hydrogel transdermal patches with red onion peel extract.