The effects of dietary stachyose as prebiotic on immunity and anti-oxidant related genes' expression and lipid metabolism in zebrafish (Danio rerio)

Abstract

An 8-week feeding trial was conducted to examine the efficacy of stachyose as a prebiotic on immune parameters, antioxidant-/immune-related genes' expression, and lipid metabolism of zebrafish. Three hundred zebrafish (0.45 ± 0.08 g) were fed four diets containing different stachyose levels at 0, 1, 2 and 4 g kg-1, respectively. After eight weeks of the feeding trial, immunity, anti-oxidant defence and lipid metabolism were tested. It was observed that the addition of stachyose to the diet induced no significant influence (P>0.05) in SOD, GPX, and CAT, gene's expression, compared to the control diet. The inclusion of stachyose resulted in no significant changes in immune gene expression (Lyz, IL-1, IL-6, and TNF) in zebrafish (P>0.05) compared to the control diet. Total cholesterol, triglyceride and LDL (low-density lipoprotein) significantly (P<0.05) decreased with the addition of 2 and 4 g kg-1 stachyose, while fish fed the control diet and 1 g.kg-1 recorded the highest significant value of LDL (P<0.05). Fish fed diet, either control or diet supplemented with 0.5 g kg-1 stachyose, recorded the lowest HDL value (P<0.05) compared to other treatments. In conclusion, stachyose can be potentially used as a feed additive to modulate lipid metabolism. However, this prebiotic couldn't benefit immune parameters and anti-oxidant defence.