Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/70613
Title: Monitoring of the organophosphate pesticide chlorpyrifos in vegetable samples from local markets in Northern Thailand by developed immunoassay
Authors: Surat Hongsibsong
Tippawan Prapamontol
Ting Xu
Bruce D. Hammock
Hong Wang
Zi Jian Chen
Zhen Lin Xu
Authors: Surat Hongsibsong
Tippawan Prapamontol
Ting Xu
Bruce D. Hammock
Hong Wang
Zi Jian Chen
Zhen Lin Xu
Keywords: Environmental Science;Medicine
Issue Date: 1-Jul-2020
Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Chlorpyrifos is an organophosphate pesticide that is wildly used among farmers for crop protection. However, there are concerns regarding its contamination in the environment and food chain. In the present study, an in-house indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) specific for detecting chlorpyrifos is developed and validated against gas chromatography–flame photometric detection (GC-FPD) as the conventional method. The developed ic-ELISA was used for detecting chlorpyrifos residue in vegetable samples. The developed ic-ELISA showed good sensitivity to chlorpyrifos at an IC50 of 0.80 µg/kg, with low cross-reactivity to other organophosphate pesticides. The 160 samples were collected from local markets located in the Chiang Rai, Chiang Mai, and Nan provinces in northern Thailand. The positive rate of chlorpyrifos residues in the vegetable samples was 33.8%, with the highest levels found in cucumbers, coriander, and morning glory, at 275, 145, and 35.3 µg/kg, respectively. The highest median levels of chlorpyrifos found in the detected samples were Chinese cabbage (332 µg/kg), cucumber (146.3 µg/kg) and Chinese Kale (26.95 µg/kg). The developed ic-ELISA is suitable for the rapid quantitation of chlorpyrifos residues.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087180960&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70613
ISSN: 16604601
16617827
Appears in Collections:CMUL: Journal Articles

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