Improvement of Dendranthemum grandiflora cv. canter with colchicine in vitro

Abstract

© 2020 Association of Agricultural Technology in Southeast Asia. All rights reserved. The mutation of chrysanthemum with ray florets was induced by colchicine. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/l NAA and 4 mg/l Kinetin for inducing calli for 12 weeks. The shoots were regenerated from callus when cultured on MS medium for 8 weeks. The plant growth, size of stomata and number of chloroplasts per stomata were not statistically different in each treatment. However, the ray florets were received high levels of colchicine is less to survivor rate. The plantlet of chrysanthemum was transfered to pot plant containing soil and chopped coconut. Five of ten plant showed the flowers color changed from purple into a pinkish-orange at the 0.10% colchicine for 12 hours. The explant obtained from soaked of 0.15 % colchicine for 12 hours with the highest number of flowers per plant, 72 flowers per plant.