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dc.contributor.authorSongyot Anuchapreedaen_US
dc.contributor.authorFah Chueahongthongen_US
dc.contributor.authorNatsima Viriyaadhammaaen_US
dc.contributor.authorPawaret Panyajaien_US
dc.contributor.authorRiki Anzawaen_US
dc.contributor.authorSingkome Timaen_US
dc.contributor.authorChadarat Ampasavateen_US
dc.contributor.authorAroonchai Saiaien_US
dc.contributor.authorMethee Rungrojsakulen_US
dc.contributor.authorToyonobu Usukien_US
dc.contributor.authorSiriporn Okonogien_US
dc.date.accessioned2020-04-02T15:23:35Z-
dc.date.available2020-04-02T15:23:35Z-
dc.date.issued2020-03-02en_US
dc.identifier.issn14203049en_US
dc.identifier.other2-s2.0-85081686177en_US
dc.identifier.other10.3390/molecules25061300en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081686177&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/68232-
dc.description.abstract© 2020 MDPI AG. All rights reserved. Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms' tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemistryen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleAntileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leavesen_US
dc.typeJournalen_US
article.title.sourcetitleMoleculesen_US
article.volume25en_US
article.stream.affiliationsRajabhat Universityen_US
article.stream.affiliationsSophia Universityen_US
article.stream.affiliationsChiang Mai Universityen_US
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