Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/63901
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPongrawee Nimnoien_US
dc.contributor.authorNeelawan Pongsilpen_US
dc.contributor.authorSaisamorn Lumyongen_US
dc.date.accessioned2019-05-07T09:59:37Z-
dc.date.available2019-05-07T09:59:37Z-
dc.date.issued2017en_US
dc.identifier.issn0125-2526en_US
dc.identifier.urihttp://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8265en_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/63901-
dc.description.abstractThe objectives of this study were to select effective bacteria that produced high amounts of IAA and ammonia, and to determine the potential of using agar compared with alginate and perlite as cell immobilization materials to maintain a long survival of the inoculant strains. The Ensifer spp. strains LP2/20, NK2/9 and Bacillus sp. strain NK2/17 were selected, immobilized and stored at 30 °C for 15 days. After 15 days of storage, Ensifer sp. strain LP2/20 immobilized in agar exhibited the highest number of bacterial survival which was significantly different from those of other materials. In pot experiments, the establishment and activity of the inoculant after 15 days of introducing into soil were investigated by PCR-denaturing gradient gel electrophoresis (DGGE) and Reverse Transcription (RT) PCR-DGGE. Cloning and sequencing of 16S rRNA gene fragments obtained from DNA- and RNA-based DGGE gels confirmed that agar and alginate were the preferable immobilization materials that prolonged the survival times and supported the establishment of the inoculant after being introduced into soil rather than absorption in perlite. The inoculant immobilized in agar and alginate were still metabolically active after 15 days of introducing into soil, while usage of perlite as a carrier and liquid inoculation were proved ineffective in maintaining the activity of the inoculant. Moreover, inoculation with either immobilization material exhibited the potential to change bacterial community structure in soil.en_US
dc.languageEngen_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleThe Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soilen_US
dc.typeบทความวารสารen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume44en_US
article.stream.affiliationsDepartment of Microbiology, Faculty of Liberal Arts and Sciences, Kasetsart University, Nakhon Pathom 73140, Thailand.en_US
article.stream.affiliationsDepartment of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.en_US
article.stream.affiliationsMicrobiology Division, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.en_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.