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dc.contributor.authorNalin Wongkattiyaen_US
dc.contributor.authorIan H. Fraseren_US
dc.contributor.authorPiyanuch Niamsupen_US
dc.contributor.authorPhanchana Sanguansermsrien_US
dc.contributor.authorDonruedee Sanguansermsrien_US
dc.description.abstractThis aims of this report were to evaluate Streptococcus bovis RMM 902 as a bacteriocin producer, to carry out partial characterization and purification of the bacteriocin, and to investigate its antibacterial activity against variety of pathogenic bacteria. A bacteriocin producer strain was evaluated for antibacterial activity against a variety of pathogenic bacteria. Effects of temperature and pH on bacteriocin activity were studied. The bacteriocin was purified by ammonium sulfate precipitation and ion exchange chromatography. The bacteriocin activity was tested at each step of purification. The inhibitory activity of Streptococcus bovis RMM 902 was demonstrated against some Gram positive and Gram negative bacteria such as S. dysgalactiae, Listeria monocytogenes, Salmonella typhi and Salmonella enteritidis. The inhibitory activity was presented over a broad range of pH and tolerant to high temperature. Purification with ammonium sulfate precipitation increased the antibacterial activity from 80 to 640 AU/ml with a 5.3 fold purification factor. Ion exchange chromatography increased purification factor to 35.5 fold and the bacteriocin was sized at less than 3,400 Da. The bacteriocin isolated from Streptococcus bovis RMM 902 was shown to provide effective control on pathogenic bacteria. There is the potential to use this bacteriocin for the prevention of bovine mastitis caused by S. dysgalactiae and potentially could be used as a food preservative.en_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleProduction and Characterization of Bacteriocin-producing Bacteria Capable of Inhibition of Streptococcus dysgalactiaeen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume44en_US in Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand.en_US of Chemistry, Monash University, Clayton, Victoria 3800, Australiaen_US of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailanden_US of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.en_US of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.en_US
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