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dc.contributor.authorWoottichai Nachaiwiengen_US
dc.contributor.authorApinun Kanpiengjaien_US
dc.contributor.authorTakashi Watanabeen_US
dc.contributor.authorChartchai Khanongnuchen_US
dc.description.abstractFour saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate.en_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleInfluences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganismsen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume44en_US of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Muang, Chiang Mai 50100, Thailand.en_US Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto 611-0011 Japan.en_US
Appears in Collections:CMUL: Journal Articles

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