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dc.contributor.authorThongchai Taechowisanen_US
dc.contributor.authorChunhua Luen_US
dc.contributor.authorYuemao Shenen_US
dc.contributor.authorSaisamorn Lumyongen_US
dc.description.abstractStreptomyces aureofaciens CMUAc130 was isolated from the root tissue of Zingiber officinale Rosc. (Zingiberaceae). It was an antagonist of Colletotrichum musae and Fusarium oxysporum, the causative agents of anthracnose of banana and wilt of wheat, respectively. Evidence for the in vitro antibiosis of S. aureofaciens CMUAc130 was demonstrated by the zone of fungal-growth inhibition. Microscopic observations showed thickness and bulbous structures at the edges of the inhibited fungal hyphae. The culture filtrate and crude extract from this strain were all inhibitory to tested phytopathogenic fungi. The major active ingredients from the culture filtrate of S. aureofaciens CMUAc130 were purified by silica gel-column chromatography and identified to be (i) 5,7-dimethoxy-4-p-methoxylphenylcoumarin and (ii) 5,7-dimethoxy-4-phenylcoumarin by NMR and mass-spectral data, respectively. Bioassay studies showed that compounds (i) and (ii) had antifungal activities against tested fungi, and their MICs were found to be 120 and 150 μg ml-1, respectively. This is the first report of compounds (i) and (ii) from micro-organisms as active ingredients for the control of phytopathogenic fungi. © 2005 SGM.en_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSecondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activityen_US
article.volume151en_US Universityen_US Universityen_US Institute of Botany Chinese Academy of Sciencesen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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