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dc.contributor.authorNicole Ngo-Giang-Huongen_US
dc.contributor.authorWoottichai Khamduangen_US
dc.contributor.authorBaptiste Leurenten_US
dc.contributor.authorIntira Collinsen_US
dc.contributor.authorIssaren Nantasenen_US
dc.contributor.authorPranee Leechanachaien_US
dc.contributor.authorWasna Sirirungsien_US
dc.contributor.authorAram Limtrakulen_US
dc.contributor.authorTasana Leusareeen_US
dc.contributor.authorAnne Marie Comeauen_US
dc.contributor.authorMarc Lallemanten_US
dc.contributor.authorGonzague Jourdainen_US
dc.description.abstractIn resource-limited settings, most perinatally HIV-1-infected infants do not receive timely antiretroviral therapy because early HIV-1 diagnosis is not available or affordable. Objective: To assess the performance of a low-cost in-house realtime polymerase chain reaction (PCR) assay to detect HIV-1 DNA in infant dried blood spots (DBS). Methods: One thousand three hundred nineteen DBS collected throughout Thailand from non-breast-fed infants born to HIV-1-infected mothers were shipped at room temperature to a central laboratory. In-house real-time DNA PCR results were compared with Roche Amplicor HIV-1 DNA test (Version 1.5) results. In addition, we verified the Roche test performance on DBS sampled from 1218 other infants using as reference HIV serology result at 18 months of age. Results: Real-time DNA PCR and Roche DNA PCR results were 100% concordant. Compared with HIV serology results, the Roche test sensitivity was 98.6% (95% confidence interval: 92.6% to 100.0%) and its specificity at 4 months of age was 99.7% (95% confidence interval: 99.2% to 99.9%). Conclusions: In-house real-time PCR performed as well as the Roche test in detecting HIV-1 DNA on DBS in Thailand. Combined use of DBS and real-time PCR assays is a reliable and affordable tool to expand access to early HIV-1 diagnosis in remote and resourcelimited settings, enabling timely treatment for HIV-1-infected infants. Copyright © 2008 Lippincott Williams & Wilkins.en_US
dc.titleEarly HIV-I diagnosis using in-house real-time PCR amplification on dried blood spots for infants in remote and resource-limited settingsen_US
article.title.sourcetitleJournal of Acquired Immune Deficiency Syndromesen_US
article.volume49en_US of research for development, Thailanden_US Mai Universityen_US School of Public Healthen_US Provincial Hospitalen_US Ministry of Public Healthen_US of Massachusetts Bostonen_US
Appears in Collections:CMUL: Journal Articles

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