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dc.contributor.authorNobuaki Shimizuen_US
dc.contributor.authorAtsushi Tanakaen_US
dc.contributor.authorTakahisa Morien_US
dc.contributor.authorTakahiro Ohtsukien_US
dc.contributor.authorAliful Hoqueen_US
dc.contributor.authorAtsushi Jinno-Oueen_US
dc.contributor.authorChatchawann Apichartpiyakulen_US
dc.contributor.authorShigeru Kusagawaen_US
dc.contributor.authorYutaka Takebeen_US
dc.contributor.authorHiroo Hoshinoen_US
dc.date.accessioned2018-09-10T03:43:21Z-
dc.date.available2018-09-10T03:43:21Z-
dc.date.issued2008-06-25en_US
dc.identifier.issn17424690en_US
dc.identifier.other2-s2.0-47749112106en_US
dc.identifier.other10.1186/1742-4690-5-52en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47749112106&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/60471-
dc.description.abstractBackground: More than 10 members of seven-transmembrane G protein-coupled receptors (GPCRs) have been shown to work as coreceptors for human immunodeficiency virus type 1 (HIV-1), HIV type 2 (HIV-2), and simian immunodeficiency viruses (SIVs). As a common feature of HIV/SIV coreceptors, tyrosine residues are present with asparagines, aspartic acids or glutamic acids in the amino-terminal extracellular regions (NTRs). We noticed that a receptor for N-formylpeptides, FPRL1, also contains two tyrosine residues accompanied by glutamic acids in its NTR. It was reported that monocytes expressing CCR5 and FPRL1 in addition to CD4 are activated by treatment with ligands or agonists of FPRL1. Activated monocytes down-modulate CCR5 and become resistant to infection by HIV-1 strains. Thus, FPRL1 plays important roles in protection of monocyptes against HIV-1 infection. However, its own coreceptor activity has not been elucidated yet. In this study, we examined coreceptor activities of FPRL1 for HIV/SIV strains including primary HIV-1 isolates. Results: A CD4-transduced human glioma cell line, NP-2/CD4, is strictly resistant to HIV/SIV infection. We have reported that when NP-2/CD4 cells are transduced with a GPCR having coreceptor activity, the cells become susceptible to HIV/SIV strains. When NP-2/CD4 cells were transduced with FPRL1, the resultant NP-2/CD4/FPRL1 cells became markedly susceptible to some laboratory-adapted HIV/SIV strains. We found that FPRL1 is also efficiently used as a coreceptor by primary HIV-1 isolates as well as CCR5 or CXCR4. Amino acid sequences linked to the FPRL1 use could not be detected in the V3 loop of the HIV-1 Env protein. Coreceptor activities of FPRL1 were partially blocked by the forymyl-Met-Leu-Phe (fMLF) peptide. Conclusion: We conclude that FPRL1 is a novel and efficient coreceptor for HIV/SIV strains. FPRL1 works as a bifunctional factor in HIV-1 infection. Namely, the role of FPRL1 in HIV-1 infection is protective and/or promotive in different conditions. FPRL1 has been reported to be abundantly expressed in the lung, spleen, testis, and neutrophils. We detected mRNA expression of FPRL1 in 293T (embryonal kidney cell line), C8166 (T cell line), HOS (osteosarcoma cell line), Molt4#8 (T cell line), U251MG (astrocytoma cell line), U87/CD4 (CD4-transduced glioma cell line), and peripheral blood lymphocytes. Roles of FPRL1 in HIV-1 infection in vivo should be further investigated. © 2008 Shimizu et al; licensee BioMed Central Ltd.en_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleA formylpeptide receptor, FPRL1, acts as an efficient coreceptor for primary isolates of human immunodeficiency virusen_US
dc.typeJournalen_US
article.title.sourcetitleRetrovirologyen_US
article.volume5en_US
article.stream.affiliationsGunma University Faculty of Medicineen_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsNational Institute of Infectious Diseasesen_US
Appears in Collections:CMUL: Journal Articles

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