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dc.contributor.authorSriyam Supawadeeen_US
dc.contributor.authorSupachok Sinchaikulen_US
dc.contributor.authorPayungsak Tantipaiboonwongen_US
dc.contributor.authorSuree Phutrakulen_US
dc.contributor.authorShui Tein Chenen_US
dc.description.abstractDeglycosylation with anhydrous hydrogen fluoride (HF) is an alternative method for removing oligosaccharides from glycoproteins which can be extremely useful for identification of proteins and the biological roles of post-translational modifications. In this study, a glycosylated proteome of human serum was treated by anhydrous HF to deglycosylate the number of oligosaccharides from glycoproteins which facilitated protein identification using proteomic analysis. In the preliminary result, the high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC-MS) showed that there was no cleavage of disulfide bonds in HF-treated insulin as a negative control. The effect of HF deglycosylation on electrophoresis pattern was studied by resolving on one-dimensional (1-D) and two-dimensional (2-D) gels. Deglycosylation of glycoproteins in human serum resulted in different protein patterns on 1-DE and 2-DE gels with the clearer protein patterns and low amount of complexity. The deglycosylated serum proteins could be enriched and identified by MS analysis. Using this approach, it indicated that the proteins in human serum have some glycosylation that affected to the protein analysis and might possess the diverse biological functions. Therefore, this deglycosylation technique is an effective method to solve the problem of oligosaccharide interference in proteome analysis and be able to use for further glycan analysis.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMaterials Scienceen_US
dc.subjectPhysics and Astronomyen_US
dc.titleProteomic analysis of deglycosylated Proteins in normal human serum using anhydrous hydrogen fluoride treatmenten_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume35en_US Mai Universityen_US Sinica Taiwanen_US Fah Luang Universityen_US Taiwan Universityen_US
Appears in Collections:CMUL: Journal Articles

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