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Title: Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
Authors: Pheravut Wongsawad
Chalobol Wongsawad
Authors: Pheravut Wongsawad
Chalobol Wongsawad
Keywords: Medicine
Issue Date: 1-Sep-2009
Abstract: Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs.
ISSN: 01251562
Appears in Collections:CMUL: Journal Articles

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