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dc.contributor.authorWilaiwan Phakthongen_US
dc.contributor.authorGillian M. Greenwayen_US
dc.contributor.authorNicole Pammeen_US
dc.contributor.authorBoonsom Liawruangrathen_US
dc.contributor.authorSaisunee Liawruangrathen_US
dc.date.accessioned2018-09-05T03:30:49Z-
dc.date.available2018-09-05T03:30:49Z-
dc.date.issued2017-01-01en_US
dc.identifier.issn01252526en_US
dc.identifier.other2-s2.0-85010792492en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010792492&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/56831-
dc.description.abstract© 2017, Chiang Mai University. All rights reserved. A magnetic particles-based chemiluminescence immunoassay was investigated for progesterone detection by using luminometer. In this work, progesterone was determined based on the competitive binding between progesterone in the sample and progesterone-horseradish peroxidase (HRP) conjugate for a constant amount of rabbit anti-progesterone. Initially, anti-rabbit IgG coated magnetic particles conjugated with primary progesterone antibody were bound to progesterone in the samples. Then, the amount of progesterone was quantified by reacting with the residual unoccupied antibody sites with HRP-progesterone, followed by HRP substrate (luminol, H2O2, and p-iodophenol (PIP)) and finally detection of the generated chemiluminescence by a luminometer. The intensity of the emitting light was proportional to the amount of enzyme present (HRP-progesterone) and was inversely related to the amount of unlabeled progesterone in the sample. The optimum conditions for determination of progesterone were obtained at 0.15 μg L-1magnetic particles, 5.0x10-4 mol L-1luminol, 5.0 × 10-3mol L-1H2O2, 1.0 × 10-3mol L-1PIP, and phosphate buffer saline buffer pH 9. The optimal dilutions of both anti-progesterone antibody and HRP-progesterone conjugate were 1:1000. The linear relationship between chemiluminescence intensity (RLU) and various concentrations of progesterone was over the concentration range of 0.5-50.0 μg L-1. This proposed method had been successfully applied to the evaluation of progesterone in human sera.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemistryen_US
dc.subjectMaterials Scienceen_US
dc.subjectMathematicsen_US
dc.subjectPhysics and Astronomyen_US
dc.titleMagnetic particles-based chemiluminescence immunoassay for progesterone determinationen_US
dc.typeJournalen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume44en_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsUniversity of Hullen_US
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