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dc.contributor.authorSiripong Paleeen_US
dc.contributor.authorNattayaporn Apaijaien_US
dc.contributor.authorKrekwit Shinlapawittayatornen_US
dc.contributor.authorSiriporn C. Chattipakornen_US
dc.contributor.authorNipon Chattipakornen_US
dc.description.abstract© 2016 The Author(s) Published by S. Karger AG, Basel. Background/Aims: Oxidative stress induced intracellular Ca2+overload plays an important role in the pathophysiology of several heart diseases. Acetylcholine (ACh) has been shown to suppress reactive oxygen species generation during oxidative stress. However, there is little information regarding the effects of ACh on the intracellular Ca2+regulation in the presence of oxidative stress. Therefore, we investigated the effects of ACh applied before or after hydrogen peroxide (H2O2) treatment on the intracellular Ca2+regulation in isolated cardiomyocytes. Methods: Single ventricular myocytes were isolated from the male Wistar rats for the intracellular Ca2+transient study by a fluorimetric ratio technique. Results: H2O2significantly decreased both of intracellular Ca2+transient amplitude and decay rate. ACh applied before, but not after, H2O2treatment attenuated the reduction of intracellular Ca2+transient amplitude and decay rate. Both atropine (a muscarinic acetylcholine receptor blocker) and mecamylamine (a nicotinic acetylcholine receptor blocker) significantly decreased the protective effects of acetylcholine on the intracellular Ca2+regulation. Moreover, the combination of atropine and mecamylamine completely abolished the protective effects of acetylcholine on intracellular Ca2+transient amplitude and decay rate. Conclusion: ACh pretreatment attenuates H2O2-induced intracellular Ca2+dyshomeostasis through both muscarinic and nicotinic receptors.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAcetylcholine Attenuates Hydrogen Peroxide-Induced Intracellular Calcium Dyshomeostasis Through Both Muscarinic and Nicotinic Receptors in Cardiomyocytesen_US
article.title.sourcetitleCellular Physiology and Biochemistryen_US
article.volume39en_US Fah Luang Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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