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dc.contributor.authorKitiphong Khongphinitbunjongen_US
dc.contributor.authorLilia I. de Guzmanen_US
dc.contributor.authorMatthew R. Tarveren_US
dc.contributor.authorThomas E. Rindereren_US
dc.contributor.authorYanping Chenen_US
dc.contributor.authorPanuwan Chantawannakulen_US
dc.description.abstract© 2014 Elsevier Ltd. The viral levels and immune responses of Italian honey bees (IHB), Russian honey bees (RHB) and an outcross of Varroa Sensitive Hygienic bees (POL) deliberately infested with one or two foundress Varroa were compared. We found that the Deformed wing virus (DWV) level in IHB inoculated with one or two foundress Varroa increased to about 103or 105fold the levels of their uninfested brood. In contrast, POL (102or 104fold) and RHB (102or l04fold) supported a lower increase in DWV levels. The feeding of different stages of Varroa nymphs did not increase DWV levels of their pupal hosts. Analyses of their corresponding Varroa mites showed the same trends: two foundress Varroa yielded higher DWV levels than one foundress, and the addition of nymphs did not increase viral levels. Using the same pupae examined for the presence of viruses, 16 out of 24 genes evaluated showed significant differential mRNA expression levels among the three honey bee stocks. However, only four genes (Defensin, Dscam, PPOact and spaetzle), which were expressed at similar levels in uninfested pupae, were altered by the number of feeding foundress Varroa and levels of DWV regardless of stocks. This research provides the first evidence that immune response profiles of different honey bee stocks are induced by Varroa parasitism.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleDifferential viral levels and immune gene expression in three stocks of Apis mellifera induced by different numbers of Varroa destructoren_US
article.title.sourcetitleJournal of Insect Physiologyen_US
article.volume72en_US Mai Universityen_US Agricultural Research Service, Washington DCen_US ARS Beltsville Agricultural Research Centeren_US
Appears in Collections:CMUL: Journal Articles

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