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dc.contributor.authorJ. Tangtuaen_US
dc.contributor.authorC. Techapunen_US
dc.contributor.authorR. Pratanaphonen_US
dc.contributor.authorA. Kuntiyaen_US
dc.contributor.authorV. Sanguanchaipaiwongen_US
dc.contributor.authorT. Chaiyasoen_US
dc.contributor.authorP. Hanmuangjaien_US
dc.contributor.authorP. Seesuriyachanen_US
dc.contributor.authorN. Leksawasdien_US
dc.date.accessioned2018-09-04T10:07:06Z-
dc.date.available2018-09-04T10:07:06Z-
dc.date.issued2015-01-01en_US
dc.identifier.issn15882535en_US
dc.identifier.issn01393006en_US
dc.identifier.other2-s2.0-84946574166en_US
dc.identifier.other10.1556/066.2015.44.0015en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84946574166&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/54053-
dc.description.abstract© 2015 Akadémiai Kiadó, Budapest. Candida tropicalis TISTR 5350 was used in the comparison of seven concentration levels of silver nanoparticles (0, 5, 10, 15, 20, 25, and 30 μg ml-1) for cell disruption methods. The optimized cell disruption strategy was selected based on the optimal protein yield and biological activity. The maximum volumetric and specifi c pyruvate decarboxylase (PDC, EC 4.1.1.1) activities (0.53±0.05 U ml-1 and 0.17±0.02 U mg-1 protein, respectively) were observed at 15 μg ml-1 silver nanoparticles. The silver nanoparticle concentration level of 15 μg ml-1 was investigated further by comparing the reaction mixtures at different time intervals of 0, 1, 2, 3, 4, 5, and 6 min. The result showed that the highest specifi c PDC activity of 0.39±0.01 U mg-1 protein was obtained from mixing for 3 min. This was not significantly different (P≤0.05) from other mixing time intervals.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleEvaluation of cell disruption for partial isolation of intracellular pyruvate decarboxylase enzyme by silver nanoparticles methoden_US
dc.typeJournalen_US
article.title.sourcetitleActa Alimentariaen_US
article.volume44en_US
article.stream.affiliationsRajabhat Universityen_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsKing Mongkut's Institute of Technology Ladkrabangen_US
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