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dc.contributor.authorNicha Charoensrien_US
dc.contributor.authorAmporn Suphatrakulen_US
dc.contributor.authorRungtawan Sriburien_US
dc.contributor.authorThippawan Yasangaen_US
dc.contributor.authorJiraphan Junjhonen_US
dc.contributor.authorPoonsook Keelapangen_US
dc.contributor.authorUtaiwan Utaipaten_US
dc.contributor.authorChunya Puttikhunten_US
dc.contributor.authorWatchara Kasinrerken_US
dc.contributor.authorPrida Malasiten_US
dc.contributor.authorNopporn Sittisombuten_US
dc.description.abstractRecombinant virus-like particles (rVLPs) of flaviviruses are non-infectious particles released from cells expressing the envelope glycoproteins prM and E. Dengue virus rVLPs are recognized as a potential vaccine candidate, but large scale production of these particles is hindered by low yields and the occurrence of cytopathic effects. In an approach to improve the yield of rVLPs from transfected insect cells, several components of a dengue serotype 2 virus prM. +. E expression cassette were modified and the effect of these modifications was assessed during transient expression. Enhancement of extracellular rVLP levels by simultaneous substitutions of the prM signal peptide and the stem-anchor region of E with homologous cellular and viral counterparts, respectively, was further augmented by codon optimization. Extensive formation of multinucleated cells following transfection with the codon-optimized expression cassette was abrogated by introducing an E fusion loop mutation. This mutation also helped restore the extracellular E levels affected negatively by alteration of a charged residue at the pr-M junction, which was intended to promote maturation of rVLPs during export. Optimized expression cassettes generated in this multiple add-on modification approach should be useful in the generation of stably expressing clones and production of dengue virus rVLPs for immunogenicity studies. © 2014 Elsevier B.V.en_US
dc.subjectImmunology and Microbiologyen_US
dc.titleAn optimized expression vector for improving the yield of dengue virus-like particles from transfected insect cellsen_US
article.title.sourcetitleJournal of Virological Methodsen_US
article.volume205en_US Kaen Universityen_US National Center for Genetic Engineering and Biotechnologyen_US Mai Universityen_US Universityen_US
Appears in Collections:CMUL: Journal Articles

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