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Title: A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
Authors: Supansa Pata
Saichit Khummuang
Sakorn Pornprasert
Thanusak Tatu
Watchara Kasinrerk
Keywords: Biochemistry, Genetics and Molecular Biology
Health Professions
Immunology and Microbiology
Issue Date: 3-Apr-2014
Abstract: Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC.
ISSN: 15324230
Appears in Collections:CMUL: Journal Articles

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