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dc.contributor.authorWasin Charerntantanakulen_US
dc.contributor.authorSurangkanang Yamkanchooen_US
dc.contributor.authorWatchara Kasinrerken_US
dc.date.accessioned2018-09-04T09:28:55Z-
dc.date.available2018-09-04T09:28:55Z-
dc.date.issued2013-03-18en_US
dc.identifier.issn18732534en_US
dc.identifier.issn01652427en_US
dc.identifier.other2-s2.0-84884728296en_US
dc.identifier.other10.1016/j.vetimm.2013.02.013en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884728296&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/52648-
dc.description.abstractPorcine reproductive and respiratory syndrome virus (PRRSV) suppresses the pro-inflammatory immune response following infection of myeloid antigen-presenting cells. A reduced pro-inflammatory immune response modulates PRRSV replication, clinical disease, and persistent infection of the virus. Numerous efforts have been made to enhance the pro-inflammatory immune response to PRRSV, but only a few attempts have so far elicited satisfactory results. The present study aims to evaluate in vitro the potential of plasmids expressing porcine interferon gamma (pcDNA-IFNγ) to enhance the expression of pro-inflammatory immune parameters in PRRSV-inoculated monocytes. Naïve blood monocytes from eight PRRSV-seronegative pigs were inoculated with PRRSV and subsequently transfected with pcDNA-IFNγ or pcDNA (empty plasmid vector) and stimulated with lipopolysaccharide (LPS). The mRNA expression levels of IFNγ, interleukin-1 beta (IL-1β), IL-10, IL-12p40, tumor necrosis factor alpha (TNFα), transforming growth factor beta (TGFβ), CD80, and CD86 were evaluated by real-time PCR. The IFNγ, IL-10, and TNFα protein production was determined by ELISA. Compared with PRRSV-inoculated monocyte control, transfection with pcDNA-IFNγ, but not pcDNA, significantly enhanced IFNγ, TNFα, CD80, and CD86 mRNA expression, and IFNγ and TNFα protein production. A slight increase in IL-1β and IL-12p40 mRNA expression was also observed. Neither pcDNA-IFNγ nor pcDNA transfection affected IL-10 and TGFβ expression. Our results thus suggest that pcDNA-IFNγ may be an effective immunostimulator for potentiating the pro-inflammatory immune response to PRRSV. © 2013 Elsevier B.V.en_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectVeterinaryen_US
dc.titlePlasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virusen_US
dc.typeJournalen_US
article.title.sourcetitleVeterinary Immunology and Immunopathologyen_US
article.volume153en_US
article.stream.affiliationsMaejo Universityen_US
article.stream.affiliationsChiang Mai Universityen_US
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