Please use this identifier to cite or link to this item:
Title: Screening of volcanic cave actinomycetes for antimicrobial activity against Paenibacillus larvae, a honey bee pathogen
Authors: Silvia Kay
Wasu Pathom-Aree
Naowarat Cheeptham
Keywords: Biochemistry, Genetics and Molecular Biology
Materials Science
Physics and Astronomy
Issue Date: 11-Feb-2013
Abstract: Actinomycete strains isolated from Helmcken Falls cave in Wells Gray Provincial Park, British Columbia were discovered to have antimicrobial activity against Paenibacillus larvae, the causative agent of American foulbrood disease in honeybees. The antimicrobial activity of 119 isolates grown in three fermentation liquid media, namely YMG, AMHU- 4 and V8 juice, at 28°C on an orbital shaker at 250 rpm for 7 days, were tested against P. larvae (ATCC 13537T) using the paper disc agar diffusion method. Of the strains screened, strain E9, grown in V-8 juice medium showed significant antibacterial activity against P. larvae with a maximum activity on day 4 of incubation at pH 7. Chemotaxonomic study and phylogenetic analysis based on 16S rRNA gene sequence revealed that strain E9 is a member of the genus Streptomyces. This is the first report on bioprospecting in volcanic caves of Canada.
ISSN: 01252526
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.