Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSutha Sangiambuten_US
dc.contributor.authorAmporn Suphatrakulen_US
dc.contributor.authorRungtawan Sriburien_US
dc.contributor.authorPoonsook Keelapangen_US
dc.contributor.authorChunya Puttikhunten_US
dc.contributor.authorWatchara Kasinrerken_US
dc.contributor.authorPrida Malasiten_US
dc.contributor.authorNopporn Sittisombuten_US
dc.description.abstractA simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cellsen_US
article.title.sourcetitleVirus Researchen_US
article.volume174en_US National Center for Genetic Engineering and Biotechnologyen_US Mai Universityen_US Universityen_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.