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Title: Flow-Batch Method with a Sequential Injection System for Spectrophotometric Determination of Selenium(IV) in Selenium-Enriched Yeast Using o-Phenylenediamine
Authors: Yaowalak Khanhuathon
Watsaka Siriangkhawut
Piyanete Chantiratikul
Kate Grudpan
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jul-2013
Abstract: A precise, accurate, and reliable flow-batch spectrophotometric method for the determination of selenium (IV) was developed using o-phenylenediamine as a reagent with a sequential injection monosegmented flow system incorporating a simple heating unit. The reaction zones of selenium(IV) and o-phenylenediamine were mixed and heated in a chamber at 62°C for 5 minutes. The piaselanol complexes were then detected at a maximum absorption wavelength of 335 nm. In-line single standard calibration and standard addition procedures were developed employing the monosegmented flow technique. Under the optimized conditions, a linear calibration graph in a range of 0.1-4.0 mg L-1selenium (IV) was obtained with limits of detection and quantitation of 0.01 and 0.1 mg L-1, respectively. Relative standard deviations were 2% [for both 0.1 and 0.5 mg L-1selenium (IV) (n = 11)]. A sample throughput of 2 h-1using four standard addition levels was achieved. The developed system was successfully applied to raw selenium-enriched yeast samples. The analyses performed by the developed method agreed well with those obtained from a standard inductively coupled plasma mass spectrometry method. © 2013 Copyright Taylor and Francis Group, LLC.
ISSN: 1532236X
Appears in Collections:CMUL: Journal Articles

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