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http://cmuir.cmu.ac.th/jspui/handle/6653943832/7586
2024-03-28T14:20:17ZDevelopment of film forming spray containing Auricularia polytricha and Agaricus bisporus extracts for skin barrier function improvement in elderly and dry skib
http://cmuir.cmu.ac.th/jspui/handle/6653943832/79279
Title: Development of film forming spray containing Auricularia polytricha and Agaricus bisporus extracts for skin barrier function improvement in elderly and dry skib
Authors: Nichcha Nitthikan
Abstract: Mushrooms are edible fruit bodies of macro-fungi that are valuable food for humans containing high amounts of essential nutrients. Edible mushrooms have secondary metabolites such as phenolic compounds, polysaccharides, and fatty acids that are interesting for application in natural cosmetic and cosmeceutical ingredients. Therefore, this study aimed to determine two different mushrooms; cloud ear mushroom (Auricularia polytricha) and portobello mushroom (Agaricus bisporus) for their attractive bioactive properties: antioxidant, anti-aging, anti-inflammatory properties, skin barrier function, and skin moisturizing effect. Then, the extracts with good bioactivities were selected to develop film forming spray for enhancing the skin barrier function and moisturized the skin. Mushrooms powder was extracted with 95%v/v ethanol to obtain cloud ear mushroom ethanol extract (CE) and portobello mushroom ethanol extract (PE). The residue mushrooms powder was then reextracted with hot water to obtain cloud ear mushroom water extract (CW) and portobello mushroom water extract (PW). The results showed that CW exhibited the highest total polysaccharide content of 748.20 ± 0.36 mg glucose/g extract. While PE and CE presented high levels of total phenolic and flavonoid contents. From HPLC analysis, the chromatograms of mushroom extracts showed the major constituents of gallic acid and ergothioneine. Gallic acid and ergothioneine were found significantly higher in PE than other extracts. Moreover, PE possessed significantly outstanding DPPH, ABTS scavenging activities, and FRAP reducing power when compare to other extracts. CW showed a high ability to inhibit hyaluronidase enzyme, whereas PE and CE presented the outstanding to delay collagen and elastin breakdown by collagenase and elastase inhibition activities. Cytotoxicity of the extracts was tested by MTT assay, all the mushroom extracts expressed more than 80%
cell viability at the highest concentration 300 μg/mL on HaCaT cells, which were non-cytotoxic. PE strongly inhibited pro-inflammation cytokine including IL-6 and TNF-α that demonstrated a skin protection effect and decreased inflammation on HaCaT cells. CW enhanced skin hydration by increasing level of filaggrin. On the other hand, PE exhibited the highest level of aquaporin-3 on HaCaT cells.
Therefore, PE was selected as a natural active ingredient to prevent inflammation, antioxidant, and anti-aging while the CW was selected as natural polymer for developing film forming spray (FFS). The FFS was formulated using 0.74%w/w CW, 1.38%w/w sodium polystyrene sulfonate, and 0.87%w/w glycerin. Film forming spray containing 0.5%w/w PE (PE-FFS) was evaluated physical appearance, drying time, occlusive factor, spray pattern, in vitro skin permeation, and hydration skin test in human volunteers. The results showed that the PE-FFS has a transparent thin film with drying time as 2.21 min and occlusive factor as 17.67%. It has a good spray pattern in spherical spots. It has good skin permeation with the long-lasting effect and increasing skin hydration after 4 weeks of application. In conclusion, the PE-FFS is a natural film forming spray formulation that can help to increase moisturizer for dry skin in elderly.2023-11-01T00:00:00ZEffects of blended vegetable and fruit juice containing probiotics on lipid levels and oxidative stress in Dyslipidemic volunteers at Bhumibol Adulyadej hospital
http://cmuir.cmu.ac.th/jspui/handle/6653943832/79093
Title: Effects of blended vegetable and fruit juice containing probiotics on lipid levels and oxidative stress in Dyslipidemic volunteers at Bhumibol Adulyadej hospital
Authors: Pattharaparn Siripun
Abstract: Dyslipidemia, a disorder of lipoprotein metabolism causing changes in blood lipid levels and leading to atherosclerosis, the main cause of cardiovascular disease, can be reduced by the intake of vegetable and fruit containing natural antioxidant, vitamins, minerals and soluble fiber. In this research, seven kinds of vegetable and fruit were selected and collected from an organic farm to make blended vegetable and fruit juice. The sensory evaluation of the blended vegetable and fruit juice from 12 formulations was performed in order to select the optimum formulation. Additionally, the soluble fiber content, total polyphenol content, and antioxidant capacity in 12 formulations are analyzed. After obtaining the optimum formulation, there was a study on the effect of the blended vegetable and fruit juice containing probiotics L. paracasei and the one without probiotics on biological markers related to dyslipidemia and oxidative stress in dyslipidemic subjects. The sensory evaluation of 12 blended vegetable and fruit juice formulations was performed to obtain the sensory responses to appearance, color, aroma, taste, texture and overall acceptability by using a 9-point Hedonic scale. The result revealed that the average sensory acceptance scores of the formulations F1 and F10 were at a high-range between 7 (like moderately) and 9 (like extremely). The formulation F10 showed the highest content of total polyphenol at 90.59±2.98 as mg Gallic acid equivalent (GAE)/100 mL of blended vegetable and fruit juice. The relation between the total polyphenol content and the antioxidant ( ABTS) values in the same formulation was detected; if the total polyphenol content was high, the antioxidant (ABTS) values were also high. The formulation F10 showed the statistically significant (p0.05). After 30 days of the clinical trial, there was a statistically non-significant difference (p>0.05) in body weight, BMI and waist circumference in both groups compared with baseline values. The systolic blood pressure of the subjects in Group 1 showed a nonsignificant decrease ( p>0.05) while the systolic blood pressure in Group 2 showed a significant decrease ( p0.05) between groups. The diastolic blood pressure of the subjects in Group 1 showed a non-significant decrease (p>0.05) while the one in Group 2 showed a significant decrease (p0.05) within group. In contrast, HDL-C, triglyceride and triglycerides/HDL-C ratio showed a statistically non-significant decrease (p>0.05) within group. The lipid profiles of the subjects in Group 2 showed a statistically significant decrease in the levels of total cholesterol, LDL-C, triglycerides and triglycerides/HDL-C ratio ( p0.05) between groups. The antioxidant capacity in plasma was measured using a modified ABTS assay and the result showed a statistically non-significant increase within group in both groups and a statistically non-significant difference (p>0.05) between groups. The lipid peroxidation in plasma which was measured using a modified MDA assay showed a statistically non-significant decrease (p>0.05) within group in Group 1. In contrast, MDA showed a significant decrease (p0.05) between groups. The oxidative stress enzymes studied in this research consisted of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutases (SOD). The three types of the oxidative stress enzyme levels in Group 1 showed a statistically non-significant increase (p>0.05) within group whereas in Group 2 showed a significant increase in catalase (CAT), glutathione peroxidase (GPx) (p0.05) in superoxide dismutases (SOD) within group while the three types of the oxidative stress enzyme levels showed a non-significant difference (p>0.05) between groups. The inflammatory marker, hs-CRP, levels in both groups showed a significant decrease (p0.05) between groups. The bile acids levels in feces of the subjects in Group1 displayed a statistically nonsignificant decrease (p>0.05) within group. In contrast, the bile acids levels in feces of the subjects in Group 2 displayed a statistically significant increase (p < 0.10) within group while the bile acids levels in feces showed a significant difference (p0.05) in lactic acid, butyric acid and propionic acid levels in feces of the subjects in Group 1 while there was a significant increase (p0.05) in lactic acid in feces of the subjects in Group 2 while there was a significant increase in acetic acid, butyric acid and propionic acid levels in feces (p0.05) between groups. According to the results obtained in this research, the blended vegetable and fruit juice formulation F10 containing L. paracasei could decrease systolic blood pressure by 9.20%, diastolic blood pressure by 10.53%, total cholesterol by 5.59%, LDL-C by 6%, triglyceride by 24.14%, triglycerides/HDL-C ratio by 30.83%, hs-CRP by 31.87%, MDA by 68.42%. In addition, it could increase HDL-C by 8.33% , catalase enzyme by 8.07% , glutathione peroxidase enzyme by 31.75% in the dyslipidemic subjects who hadn’t taken any dyslipidemic medicine at Bhumibol Adulyadej Hospital. Hence, the blended vegetable and fruit juice formulation F10 containing L. paracasei could be a new alternative method to reduce LDL-C and triglycerides and enhance primary prevention for cardiovascular diseases in dyslipidemic patients who never experience any dyslipidemic medication.2020-08-01T00:00:00ZDevelopment of nanocellulose-based hydrogels for pharmaceutical and biomedical applications
http://cmuir.cmu.ac.th/jspui/handle/6653943832/78965
Title: Development of nanocellulose-based hydrogels for pharmaceutical and biomedical applications
Authors: Pichapar O-chongpian
Abstract: Nanocellulose, derived from plants, is a sustainable material with a nanostructured composition. It exhibits a significant characteristic of high flexibility, biocompatibility, non-toxicity, and biodegradability. Moreover, the desirable properties, including surface area, pore size, and mechanical properties can be modified through various extraction and surface modification techniques applied to nanocellulose. Consequently, it is utilized in various fields, such as engineering, material reinforcement, packaging, drug delivery systems, bioactive compounds, food, pharmaceuticals, and biomedical applications. In this research, cellulose nanofibers were developed and prepared for the fabrication of hydrogels to enhance the properties of the hydrogel in order to achieve the desired characteristics. After that, the evaluation of the hydrogel properties was conducted, including its morphology, mechanical strength and flexibility, gel content, swelling behavior, drug content, in vitro drug release profile and kinetic, biocompatibility study, and antimicrobial activity, which are essential for pharmaceutical and biomedical applications. This is a pathway to enhance value and actively promote environmentally conscious green business management model, thereby facilitating the progress of a sustainable and integrated society. In the first study, hydrogel formulations were fabricated by combining cellulose nanofibers (CNFs, C), low-methoxyl pectin (LMP, P),
and sodium alginate (SA, A) at different mass ratios. Subsequently, the hydrogel was crosslinked using 3% w/w calcium chloride (Ca), 0.5 M citric acid (Ci), or a combination of 0.5 M citric acid with 3% w/w calcium chloride (Ca+Ci). The clindamycin hydrochloride was selected as the drug model to determine drug content and in vitro drug release profile and kinetic. Our results indicated that a hydrogel composed of CNFs/LMP/SA at a mass ratio of 2:0.5:0.5, crosslinked with Ca+Ci, exhibited suitable properties and showed biocompatibility with human keratinocyte cells (HaCaT). The hydrogel demonstrated a clindamycin hydrochloride content more than 80%. In vitro drug release data revealed a prolonged release profile, with a cumulative drug release percentage extending up to 3 days. This innovative hydrogel formulation shows potential for pharmaceutical applications as a transdermal drug delivery system, offering prolonged drug release for the treatment of infected wounds. However, in first study, the production of the hydrogel required the incorporation of polyethylene glycol (PEG) to enhance the dispersion of CNFs in water. To produce hydrogels with desired properties, it is necessary to mix it with other polymers. Using only CNFs for hydrogel production presents difficulties and challenges. In the second study, our objective aimed to improve the dispersion of CNFs by conducting oxidative modification on the surface of CNFs. This modification increased the presence of carboxyl groups within the CNF structure, resulting in enhanced dispersion in water. Following the assessment of the physicochemical properties, carboxylated cellulose nanofibers (c-CNFs) were obtained and used in the fabrication of polyhexamethylene biguanide (PHMB) nanocellulose hydrogels through a physical crosslinking technique. These hydrogels have been developed for antimicrobial applications in the pharmaceutical and biomedical fields. The results demonstrated that the 7% c-CNFs-2h formulation, loaded with PHMB, exhibited optimal characteristics in terms of morphology, porosity, mechanical strength, gel content and swelling behavior. Additionally, it retained over 80% of the PHMB drug content. in vitro drug release data indicated a prolonged release pattern up to 3 days. In a noteworthy achievement, this study has successfully formulated a hydrogel using only CNFs. The formulation demonstrated antimicrobial efficacy and excellent potential for pharmaceutical and biomedical applications.2023-09-01T00:00:00ZDevelopment of hydrogel containing bacterial cellulose for wound dressing application
http://cmuir.cmu.ac.th/jspui/handle/6653943832/78812
Title: Development of hydrogel containing bacterial cellulose for wound dressing application
Authors: Baramee Chanabodeechalermrung
Abstract: This study aims to develop hydrogel containing bacterial cellulose (BC) for wound dressing application. Hydrogels were prepared by dispersing BC in 40 %w/w polyethylene glycol 1500 (PEG 1500) solution after that, mixed with sodium alginate (A) solution or pectin (P) solution or sodium alginate and pectin solution, fabricated via a physical crosslinking technique using 3 %w/v calcium chloride (CaCl2) solution, and then incorporated with polyhexamethylene biguanide (PHMB), which is an effective antimicrobial drug by immersion technique. After hydrogel preparation, the physicochemical properties of all hydrogel formulations were investigated. Our results found that hydrogel incorporated sodium alginate shrunk when crosslinking with CaCl2 solution made hydrogel composites denser resulting in increasing puncture strength, and hydrogel incorporated sodium alginate without PHMB had fluff surface unlike the other formulations, that had smooth surface. Moreover, the formulations with PHMB performed better physicochemical properties than the hydrogels without PHMB. Interestingly, Fourier transform infrared spectroscopy (FT-IR) revealed the possible interaction between PHMB and the carboxylic group of sodium alginate and pectin. BC/A-PHMB hydrogel performed suitable mechanical strength, fluid uptake ability, water retention property, drug content, high integrity value, and maximum swelling degree. Additionally, in vitro cell cytotoxicity of BC/A-PHMB hydrogel revealed high biocompatibility with human keratinocyte cell line (HaCaT) and possessed prolong released of PHMB in Tris-HCl buffer pH 7.4, while rapid release in phosphate buffer saline (PBS) pH 7.4 since sodium alginate in BC/A-PHMB can bind to phosphate ion in PBS resulting in PHMB release. Moreover, BC/A-PHMB hydrogel possessed excellent anti-bacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. In conclusion, BC/A-PHMB hydrogel could be a potential dual crosslinked ion-based hydrogel for modern wound dressing with excellent anti-bacterial activity.2022-12-01T00:00:00Z